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Abstract. We present a systematic approach to minimizing the Z-domain of protein A, a three-helix bundle (59 residues total) that binds tightly (Kd = 10 nM) to the Fc portion of an immunoglobin IgG1. Despite the fact that all the contacts seen in the x-ray structure of the complex with the IgG are derived from residues in the first two helices ...Multimerization of Z C a. In order to optimize Z Ca for development of a chromatography resin with high binding capacity, three multimeric versions (di-, tri- and tetrameric) of the domain were constructed and successfully produced, all containing a C-terminal cysteine for directed coupling. As reference, the monomeric version, also equipped with a C-terminal … ….

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Oct 12, 2023 · The positive integers 1, 2, 3, ..., equivalent to N. References Barnes-Svarney, P. and Svarney, T. E. The Handy Math Answer Book, 2nd ed. Visible Ink Press, 2012 ... Fundamental domain for = SL 2(Z) on H The simplest beginning choice of discrete subgroup of G= SL 2(R) is = SL 2(Z) = f2-by-2 integer matrices with determinant 1g Both for use below and to show that SL 2(Z) is a large group, note: [2.0.1] Claim: Given relatively prime integers c;d, there are integers a;bsuch that a b

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